Incoherent color holography lattice light-sheet for subcellular imaging of dynamic structures

The purpose of the article is to explore need and advantages using incoherent color holography lattice light-sheet (ICHLLS) provide multiwavelength quantitative monitoring 3D cellular dynamics in live tissue further understand complex functions cells compartments. We have explored use investigate colocalization fluorescent markers intact tissue. Neuronal structures an attractive target for light-sheet. show a architecture space which signaling both between within subcellular requires proteins lipids function. During activity over long periods it important understanding these Parkinson’s, Alzheimer’s motoneuron diseases As proof concept this recalls key aspects imaging provides description detection system configuration actively control dual diffractive lenses phase-shifting at multiple excitation wavelengths sequentially, per each z-galvo scanning level, with extended field-of-view. will allow simultaneous recording multidimensional object waves that contain intensity space, phase, wavelength information. measure fluorescence indicators introduced into neural